Genomic Copy Number Analysis Using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design

Methods Mol Biol. 2024;2794:293-304. doi: 10.1007/978-1-0716-3810-1_24.ABSTRACTDroplet digital PCR (ddPCR) is an emerging method for the absolute quantification of PCR products, and it can detect DNA copy numbers accurately. It analyzes the end-point absolute fluorescence signals of the PCR-positive droplets and calculates the target concentration. EvaGreen is a nonspecific double-stranded DNA-binding fluorescent dye, and the ddPCR system also supports assays using this cost-effective hydrolysis probe. Here, we describe a simple method of quantification for DNA copy numbers using the EvaGreen single-color fluorescent design.PMID:38630238 | DOI:10.1007/978-1-0716-3810-1_24
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research