Complete genome sequence of a novel badnavirus infecting Fatsia japonica in China

AbstractThe complete genome sequence of a novel badnavirus, tentatively named “fatsia badnavirus 1” (FaBV1, OM540428), was identified inFatsia japonica. The infected plant displayed virus-like symptoms on leaves, including yellowing and chlorosis. The genome of FaBV1 is 7313 bp in length and similar in size and organization to other members of the genusBadnavirus (familyCaulimoviridae), containing four open reading frames (ORFs), three of which are found in all known badnaviruses, and the other of which is only present in some badnaviruses. The virus has the genome characteristics of badnaviruses, including a tRNAMet binding site (5 ’-TCTGAATTTATAGCGCTA-3’) and two cysteine-rich domains (C-X-C-2X-C-4X-H-4X-C and C-2X-C-11X-C-2X-C-4X-C-2X-C). Pairwise sequence comparisons of the RT+RNase H region indicated that FaBV1 shares 61.4-71.2% nucleotide (nt) sequence identity with other known badnaviruses, which is below the thresho ld (80% nt sequence identity in the RT+RNase H region) used for species demarcation in the genusBadnavirus. Phylogenetic analysis revealed that FaBV1, ivy ringspot-associated virus (IRSaV, MN850490.1), and cacao mild mosaic virus (CMMV, KX276640.1) together form a separate clade within the genusBadnavirus, suggesting that FaBV1 is a new member of the genusBadnavirus in the familyCaulimoviridae. To our knowledge, this is the first report of a badnavirus infectingF. japonica.

Source: Archives of Virology - April 15, 2024 Category: Virology Source Type: research