LC3B conjugation machinery promotes autophagy-independent HIV-1 entry in CD4 < sup > + < /sup > T lymphocytes

Autophagy. 2024 Apr 2. doi: 10.1080/15548627.2024.2338573. Online ahead of print.ABSTRACTHIV-1 entry into CD4+ T lymphocytes relies on the viral and cellular membranes' fusion, leading to viral capsid delivery in the target cell cytoplasm. Atg8/LC3B conjugation to lipids, process named Atg8ylation mainly studied in the context of macroautophagy/autophagy, occurs transiently in the early stages of HIV-1 replication in CD4+ T lymphocytes. Despite numerous studies investigating the HIV-1-autophagy interplays, the Atg8ylation impact in these early stages of infection remains unknown. Here we found that HIV-1 exposure leads to the rapid LC3B enrichment toward the target cell plasma membrane, in close proximity with the incoming viral particles. Furthermore, we demonstrated that Atg8ylation is a key event facilitating HIV-1 entry in target CD4+ T cells. Interestingly, this effect is independent of canonical autophagy as ATG13 silencing does not prevent HIV-1 entry. Together, our results provide an unconventional role of LC3B conjugation subverted by HIV-1 to achieve a critical step of its replication cycle.PMID:38566318 | DOI:10.1080/15548627.2024.2338573

Source: Autophagy - April 3, 2024 Category: Cytology Authors: Baptiste Pradel Guilhem Cantaloube Marie Villares Ma ïka S Deffieu V éronique Robert-Hebmann Vincent Lucansky Mathias Faure Nathalie Chazal Rapha ël Gaudin Lucile Espert Source Type: research