TFE3/PI3K/Akt/mTOR axis in renal cell carcinoma affects tumor microenvironment

The role of the PI3K/Akt pathway in renal cell carcinoma (RCC) progression, metastasis, and resistance to therapies has not been thoroughly investigated. Transcription factor E3 (TFE3) expression is related to poorer prognosis and tumor microenvironment in patients with RCC. We aimed to determine the relationship between TFE3 and the PI3K/Akt pathway. TFE3 downregulation was achieved by transient transfection of small interfering RNA (siRNA) and short hairpin RNA (shRNA) in UOK146 cells. TFE3 overexpression was induced by transient transfection with pcDNA3.1 encoding the constitutively active form of TFE3.

Source: American Journal of Pathology - April 6, 2024 Category: Pathology Authors: Chung Su Hwang, Yun Kyung Kang, Ji Yun Kim, So Hyun Shin, Joon Young Park, Ji Sun Song, So Young Kim, Se Jin Jung, Jung Hee Lee, Ju-Young Na, Dong Hoon Shin, Jee Yeon Kim, Sung Woo Park, Hyun Jung Lee Tags: Regular Article Source Type: research