Primer exchange reaction-coupled transcription isothermal amplification as a sensitive biomolecular assay

Chem Commun (Camb). 2024 Apr 4. doi: 10.1039/d4cc00665h. Online ahead of print.ABSTRACTWe devised a novel strategy that relies on a combination of the primer exchange reaction (PER) with transcription isothermal amplification, termed PER-Trap, for a sensitive biomolecular assay. Its design allowed light-up RNA aptamers to be produced as the final product, leading to the generation of an amplified fluorescence signal. The utility of PER-Trap was successfully demonstrated by the detection of exosomes.PMID:38572617 | DOI:10.1039/d4cc00665h

Source: Chemical Communications - April 4, 2024 Category: Chemistry Authors: Jinseo Son Seok Hyeon Kim Byung Seok Cha Eun Sung Lee Seokjoon Kim Ki Soo Park Source Type: research