A cell-permeable Ub-Dha probe for profiling E1-E2-E3 enzymes in live cells

Chem Commun (Camb). 2024 Mar 28. doi: 10.1039/d4cc00415a. Online ahead of print.ABSTRACTActivity-based ubiquitin probes (Ub-ABPs) have recently been developed as effective tools for studying the capabilities of E1-E2-E3 enzymes, but most of them can only be used in cell lysates. Here, we report the first cell-penetrating Ub-Dha probes based on thiazolidine-protected cysteines, which enable successful delivery into cells confirmed by a fluorophore at the N-terminus of Ub and live-cell fluorescence microscopy. A total of 18 E1-E2-E3 enzymes in live cells were labelled and enriched in combination with label-free quantification (LFQ) mass spectrometry. This work provided a new cell-penetrating Ub tool for studying the activity and function of Ub-related enzymes.PMID:38545842 | DOI:10.1039/d4cc00415a

Source: Chemical Communications - March 28, 2024 Category: Chemistry Authors: Qiong Xia Xianbin Meng Yu Wang Rujing Yuan Pincheng Li Liwen Liu Yi-Ming Li Source Type: research