Local administration of myeloid-derived suppressor cells prevents progression of immune-mediated dry eye disease

Exp Eye Res. 2024 Mar 23:109871. doi: 10.1016/j.exer.2024.109871. Online ahead of print.ABSTRACTMyeloid derived suppressor cells (MDSCs) are a heterogenous population of immature hematopoietic precursors with known immunoregulatory functions. The immunosuppressive role of MDSCs has been highlighted in several inflammatory ophthalmic disorders; however, their therapeutic application in suppressing the immune-mediated changes in dry eye disease (DED) have not been studied. We observed significant reduction in antigen presenting cell (APC) frequencies and their maturation in the presence of MDSCs. Moreover, co-culturing MDSCs with T helper 17 cells (Th17) resulted in reduced Th17 frequencies and their IL-17 expression. On the contrary, MDSCs maintained regulatory T cell frequencies and enhanced their function in-vitro. Furthermore, we delineated the role of interleukin (IL-10) secreted by MDSCs in their immunoregulatory functions. We confirmed these results by flow cytometry analysis and observed that treatment with MDSCs in DED mice effectively suppressed the maturation of APCs, pathogenic Th17 response, and maintained Treg function and significantly ameliorated the disease. The results in this study highlight the potential therapeutic application of MDSCs in treating refractory DED.PMID:38527580 | DOI:10.1016/j.exer.2024.109871

Source: Experimental Eye Research - March 25, 2024 Category: Opthalmology Authors: Pier Luigi Surico Seokjoo Lee Rohan Bir Singh Amirreza Naderi Shilpy Bhullar Tomas Blanco Yihe Chen Reza Dana Source Type: research