Pharmacokinetic evaluation of a single chain antibody fragment against scorpion toxins in sheep

Toxicon. 2024 Mar 22:107691. doi: 10.1016/j.toxicon.2024.107691. Online ahead of print.ABSTRACTA key aspect during the development of antivenoms is the evaluation of the efficiency and security of the therapeutic molecules. In this work, we report the pharmacokinetic analysis of a neutralizing single chain antibody fragment named LR (scFv LR) where three sheep were used as a large animal model. The animals were injected through i.v. route with 2 mg of scFv LR. Blood samples were drawn every minute within the first 15 min, the sampling continues at 20, 25, 30, 45, 60, 90, 120 min, subsequently at one-hour intervals, 3, 4, 5, 6 h, two more samples at 9 and 12 h and, two more samples at 24 and 48 hours and finally at one-day intervals during 4 days. scFv LR levels were measured from blood serum and urine samples by an ELISA. The pharmacokinetics of the experimental data was analyzed using the three-exponential kinetics. The value of the fast initial component (τ1=0.409±0.258min) indicated that the scFv is distributed rapidly into the tissues. The mean residence time, MRT, was 45 ± 0.51 min and the clearance (CL), 114.3 ± 14.3 mL/min. From urine samples it was possible to detect significant amounts of scFv LR, which is evidence of renal elimination.PMID:38522587 | DOI:10.1016/j.toxicon.2024.107691

Source: Toxicon - March 24, 2024 Category: Toxicology Authors: Roberto Olivares Hern ández Lidia Ria ño Umbarila Baltazar Becerril Alejandro Alag ón Cano Hilda Vazquez Lopez Source Type: research