Structure-Based Site-Directed Mutagenesis of Hydroxynitrile Lyase from Cyanogenic Millipede, Oxidus gracilis for hydrocyanation and Henry reactions

Chembiochem. 2024 Mar 25:e202400118. doi: 10.1002/cbic.202400118. Online ahead of print.ABSTRACTHydroxynitrile lyase (HNL) from cyanogenic millipede Oxidus gracillis (OgraHNL) has been discovered as a crucial enzyme involving in the cyanogenesis pathway. Here, the crystal structures of OgraHNL complexed with sulfate, benzaldehyde (BA), (R)-Man, (R)-2-Cl-Man, and acetone cyanohydrin (ACN) were solved at 1.6, 1.7, 2.3, 2.1, and 2.0 Å resolutions, respectively. The structure of OgraHNL revealed that it belongs to the lipocalin superfamily. Based on the structure, positive mutants have been designed to further improve the catalytic activity and enantioselectivity of the enzyme on asymmetric hydrocyanation and Henry reactions.PMID:38526556 | DOI:10.1002/cbic.202400118

Source: Chembiochem - March 25, 2024 Category: Biochemistry Authors: Siriporn Chaikaew Yukio Watanabe Daijun Zheng Fumihiro Motojima Takuya Yamaguchi Yasuhisa Asano Source Type: research