Elastin-like Peptide as a Model for Disordered Proteins: Diffusion Behaviour in Self-Crowding Conditions

This study investigates the effect of crowding on the diffusion behaviour of an elastin-like peptide (ELP), by combined pulse field gradient (PFG) and static field gradient (SFG) NMR techniques. We interpret our findings in terms of highly dynamic chain assemblies with weak interactions, resulting in ELP diffusion that is primarily governed by the viscous flow of the solvent. The diffusion behaviour of the peptide appears to resemble that of globular proteins rather than flexible linear polymers over a wide concentration range.PMID:38511646 | DOI:10.1002/cphc.202400117

Source: Chemphyschem - March 21, 2024 Category: Chemistry Authors: Susann Wei ßheit Bj örn Kuttich Michael Vogel Christina M Thiele Source Type: research