Usefulness of sulfonated azo dyes to evaluate macromolecularly oriented protein substrates

Acta Histochem. 2024 Mar 12;126(3):152154. doi: 10.1016/j.acthis.2024.152154. Online ahead of print.ABSTRACTSulfonated azo dyes are crucial for the histochemical, topochemical, and electrophoretic demonstration of proteins. Additionally, these dyes may reveal the significance of evaluating the anisotropic phenomenon of linear dichroism in macromolecularly oriented stained proteins. However, this requires that the ordered -NH3+ groups available for electrostatic binding of the -SO3- dye groups are present in the protein substrate. Further, the reactive -SO3- dye groups should be positioned in a way to permit selective absorption of polarized light at the level of the dye -NN- chromophore azo groups. This review reports the usefulness of sulfonated azo dyes in revealing the extrinsic phenomenon of linear dichroism in dye-substrate complexes and changes in the oriented state of protein macromolecules.PMID:38479076 | DOI:10.1016/j.acthis.2024.152154

Source: Acta Histochemica - March 13, 2024 Category: Biochemistry Authors: Maria Luiza S Mello Eli H M Dos Anjos Benedicto de Campos Vidal Source Type: research