Improving saccharification efficiency of corn stover through ferric chloride-deep eutectic solvent pretreatment

Bioresour Technol. 2024 Mar 11:130579. doi: 10.1016/j.biortech.2024.130579. Online ahead of print.ABSTRACTAn effective deep eutectic solvent (DES) and Iron(III) chloride (FeCl3) combination pretreatment system was developed to improve the removal efficiency of lignin and hemicellulose from corn stover (CS) and enhance its saccharification. N-(2-hydroxyethyl)ethylenediamine (NE) was selected as the hydrogen-bond-donor for preparing ChCl-based DES (ChCl:NE), and a mixture of ChCl:NE (60 wt%) and FeCl3 (0.5 wt%) was utilized for combination pretreatment of CS at 110 ℃ for 50 min. FeCl3/ChCl:NE effectively removed lignin (87.0 %) and xylan (55.9 %) and the enzymatic hydrolysis activity of FeCl3/ChCl:NE-treated CS was 5.5 times that of CS. The reducing sugar yield of pretreated CS was 98.6 %. FeCl3/ChCl:NE significantly disrupted the crystal structure of cellulose in CS and improved the removal of lignin and hemicellulose, enhancing the conversion of cellulose and hemicellulose into monomeric sugars. Overall, this combination of FeCl3 and DES pretreatment methods has high application potential for the biological refining of lignocellulose.PMID:38479628 | DOI:10.1016/j.biortech.2024.130579

Source: Bioresource Technology - March 13, 2024 Category: Biotechnology Authors: Danping Zhang Jia Liu Haixu Xu Hanxiao Liu Yu-Cai He Source Type: research