Separation of crude methanol extract of < em > Plantago major < /em > root: identification of phytochemicals and assessment of biological activities

Nat Prod Res. 2024 Mar 12:1-5. doi: 10.1080/14786419.2024.2326993. Online ahead of print.ABSTRACTPlantago major root extracts were used for analysis by Gas Chromatography-Mass Spectrometry (GC-MS). The anticancer and antibacterial functions of extracts were also investigated. The dichloromethane extract of P. major had the highest inhibitory effect against Salmonella paratyphi (18.00 ± 1.4 mm) at 100 mg/mL concentration. The lowest MIC was also achieved for S. paratyphi treated with dichloromethane extract of P. major (1.5 mg/mL). The minimum MBC (2 mg/mL) was observed for dichloromethane extract of P. major root against S. paratyphi. IC50 values of dichloromethane extracts of P. major root (184.84 μg/mL) against HCT116 were lower than the ethyl acetate and butanol extracts (212.41 μg/mL and 223.93 μg/mL) at 72h. The butanol extract exhibited the most IC50 value on HEK293 (748.19 μg/mL). The biological properties of P. major extracts may be assigned to the presence of numerous compounds detected in GC/MS analysis including n-Hexadecanoic acid, Linolenic acid, Palmitic acid, methyl ester, Stearic acid.PMID:38470177 | DOI:10.1080/14786419.2024.2326993
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research