Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis

Biosci Biotechnol Biochem. 2024 Mar 8:zbae030. doi: 10.1093/bbb/zbae030. Online ahead of print.ABSTRACTFor precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry (LC-MS) analysis. Conventional fractionation using high performance liquid chromatography (HPLC) is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.PMID:38458347 | DOI:10.1093/bbb/zbae030
Source: Bioscience, Biotechnology, and Biochemistry - Category: Biochemistry Authors: Source Type: research