Measuring Ligand-bound Protein Complexes with Proximity Labeling: A Practical Guide

Chembiochem. 2024 Mar 8:e202400073. doi: 10.1002/cbic.202400073. Online ahead of print.ABSTRACTIdentifying the drug-target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. These therapies often induce changes within the cellular proteome, leading to unexpected consequences such as changes in the targets complexation state or off-target interactions between the compound and additional proteins. Currently, unbiased target-ID approaches are being used to embark on this task. Here we provide an overview of the strengths and limitations of these methods, and a practical step-by-step protocol for using the BioTAC system to assist with drug target and interactome ID.PMID:38457625 | DOI:10.1002/cbic.202400073

Source: Chembiochem - March 8, 2024 Category: Biochemistry Authors: Pavitra Goyal Andrew J Tao Elizabeth J Mumby Justin G English Fleur M Ferguson Source Type: research