Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins

Biosci Biotechnol Biochem. 2024 Mar 5:zbae026. doi: 10.1093/bbb/zbae026. Online ahead of print.ABSTRACTMethods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.PMID:38444196 | DOI:10.1093/bbb/zbae026

Source: Bioscience, Biotechnology, and Biochemistry - March 6, 2024 Category: Biochemistry Authors: Fu Kuroiwa Hiraku Suda Maho Yabuki Kimie Atsuzawa Haruhiko Yamaguchi Masatsugu Toyota Yasuko Kaneko Satoshi Yamashita Seiji Takahashi Yuzuru Tozawa Source Type: research