Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins

CONCLUSIONS: With easy manipulation, the method was effective in recycling of the selectable markers, and consequently two protein genes were sequential integrated chromosomally and successfully co-expressed in the yeast.PMID:38416308 | DOI:10.1007/s10529-024-03466-3

Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Weixian Wang Minghai Han Guofei Zhu Xiaohui Liu Tianming Zhao Xiaoyan Ma Xun Gong Cunbin Xu Source Type: research