Sulfonium-Based Activity-Based Probes for Lysine-Selective Protein Profiling under Alkaline Conditions

In this study, we constructed a series of sulfonium-based small molecules to react with the amine group of lysine residues by utilizing the strong electrophilicity of sulfonium, resulting in lysine-selective labeling via the formation of classical amide bonds under alkaline conditions (pH 9.0–11.0). After systematic optimization of the labeling conditions, this strategy was utilized for protein labeling across various bacteria’s lysates. Finally, combined with the activity-based protein profiling (ABPP) strategy, we successfully identified and analyzed hundreds of labeled lysine residues in the bacterial proteome. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, GermanyArticle in Thieme eJournals: Table of contents  |  Abstract  |  Full text

Source: Synlett - March 1, 2024 Category: Chemistry Authors: Hu, Changxing Wang, Rui An, Yuhao Wan, Chuan Yin, Feng Lu, Fei Li, Zigang Tags: letter Source Type: research