Identifying Exchangeable Protons in a 1D NMR Spectrum by Spatially Selective Exchange-Editing

Chemphyschem. 2024 Feb 26:e202300713. doi: 10.1002/cphc.202300713. Online ahead of print.ABSTRACTSignals undergoing chemical or conformational exchange in one-dimensional NMR spectra are often identified by deuterium exchange. In order to obtain quantitative information about the dynamic processes involved, one frequently used method is EXchange SpectroscopY (EXSY). To detect all exchange processes, the EXSY experiment requires the acquisition of time-consuming two-dimensional spectra. Here we report a faster alternative, an experiment which uses spatial encoding to extract similar information in a 1D exchange-edited experiment. Thereby, all protons are observed at once, but in different slices of the detection volume. The experiment can be carried out in a single scan to identify exchanging sites in a 1D spectrum by changes in signal intensity indicating exchange processes. If the exchanging partner, for example water is in molar excess the exchange-editing method easily identifies mobile protons by negative signals in the 1D 1H NMR spectrum.PMID:38407996 | DOI:10.1002/cphc.202300713

Source: Chemphyschem - February 26, 2024 Category: Chemistry Authors: Markus Rotzinger Nathalie Schuster Klaus Zangger Source Type: research