Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents

In this study, we employed the bifunctional molecule-assisted (CAET) strategy to develop a type of disulfide bond-activated heterotypic Ub reagents, which allowed to enrich heterotypic Ub-interacting proteins and modifying enzymes simultaneously. The sequential release of readers which are non-covalently bound and writers or erasers which are covalently conjugated by using urea and reductant, respectively, combined with label-free quantitative (LFQ) MS indicated that these heterotypic Ub reagents would facilitate future investigations into functional roles played by heterotypic Ub chains.PMID:38387394 | DOI:10.1016/j.bioorg.2024.107186

Source: Bioorganic Chemistry - February 22, 2024 Category: Chemistry Authors: Hongyi Cai Xiangwei Wu Junxiong Mao Zebin Tong Dingfei Yan Yicheng Weng Qingyun Zheng Source Type: research