Identification and molecular mapping of a major gene conferring resistance to Phytophthora sansomeana in soybean 'Colfax'

In this study, we crossed 'Colfax' with a susceptible parent, 'Senaki', and developed three mapping populations with a total of 234 F2:3 families. Inheritance pattern analysis indicated a 1:2:1 ratio for resistant: segregating: susceptible lines among all the three populations, indicating a single dominant gene conferring the resistance in 'Colfax' (designated as Rpsan1). Linkage analysis using extreme phenotypes anchored Rpsan1 to a 30 Mb region on chromosome 3. By selecting nine polymorphic SNP markers within the region, Rpsan1 was genetically delimited into a 21.3 cM region between Gm03_4487138_A_C and Gm03_5451606_A_C, which corresponds to a 1.06 Mb genomic region containing nine NBS-LRR genes based on Gmax2.0 assembly. The mapping results were then validated using two breeding populations derived from 'E12076T-03' × 'Colfax' and 'E16099' × 'Colfax'. Marker-assisted resistance spectrum analyses with 9 additional isolates of P. sansomeana indicated that Rpsan1 may be effective towards a broader range of P. sansomeana isolates and has strong merit in protecting soybean to this pathogen in the future.PMID:38386094 | DOI:10.1007/s00122-024-04556-6

Source: TAG. Theoretical and Applied Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: Feng Lin Muhammad Salman Zhanguo Zhang Austin G McCoy Wenlong Li Raju Thada Magar Drew Mitchell Meixia Zhao Cuihua Gu Martin I Chilvers Dechun Wang Source Type: research