Allosteric strand displacement isothermal amplification for the visual detection of Toxoplasma gondii in 30 minutes

This study established a simple, cost-effective, and rapid colorimetric detection method for T. gondii. The entire testing process, from sample collection to results, takes only 0.5 h. These characteristics fulfill the demands of researchers seeking rapid target detection with minimal equipment reliance. For genomic extraction, this study evaluated the ability of two filter papers to capture genomes. A rapid genomic extraction device combined with the two filter papers was designed to simplify the genomic extraction process, which was completed within 10 min and increased the detection sensitivity tenfold. The method utilized a simplified primer design for isothermal amplification, namely allosteric strand displacement (ASD), and employed an underutilized commercial color indicator, Bromothymol Blue (BTB), for signal output. Compared with other reported indicators, BTB exhibited a more pronounced color change, shifting from blue to yellow in positive samples, facilitating easier visual differentiation. The reaction was completed in 20 min with a limit of detection (LOD) as low as 0.014 T. gondii per microliter.PMID:38382270 | DOI:10.1016/j.bios.2024.116132

Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Lei Wang Zhaoxuan Zhang Yanger Liu Shenghao Lin Wenqiang Zhang Longjiao Zhu Wentao Xu Yulan Dong Source Type: research