Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages

We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.PMID:38361219 | DOI:10.1002/eji.202350515

Source: European Journal of Immunology - February 16, 2024 Category: Allergy & Immunology Authors: Sara J Thygesen Sabrina S Burgener Prerna Mudai Mercedes Monteleone Dave Boucher Vitaliya Sagulenko Kate Schroder Katryn J Stacey Source Type: research