A Gene-encoded Aldehyde Tag Repurposed from RiPP Cyclophane-forming Pathway

Bioorg Med Chem Lett. 2024 Feb 13:129653. doi: 10.1016/j.bmcl.2024.129653. Online ahead of print.ABSTRACTGene-encoded aldehyde tag technology has been widely utilized in protein bioorthogonal chemistry and biotechnological application. Herein, we report utilization of the promiscuous rSAM cyclophane synthase SjiB involved in triceptide biosynthesis as a dedicated and highly efficient formylglycine synthase. The new aldehyde tag sequence in this system, YQSSI, is biosynthetically orthogonal to the known aldehyde tag (C/S)x(P/A)xR. The potential use of SjiB/YQSSI aldehyde tag system was further validated in fluorescent labelling of model proteins.PMID:38360420 | DOI:10.1016/j.bmcl.2024.129653
Source: Bioorganic and Medicinal Chemistry Letters - Category: Chemistry Authors: Source Type: research