The stabilization of Arp2/3 complex generated actin filaments

Biochem Soc Trans. 2024 Jan 30:BST20230638. doi: 10.1042/BST20230638. Online ahead of print.ABSTRACTThe Arp2/3 complex, which generates both branched but also linear actin filaments via activation of SPIN90, is evolutionarily conserved in eukaryotes. Several factors regulate the stability of filaments generated by the Arp2/3 complex to maintain the dynamics and architecture of actin networks. In this review, we summarise recent studies on the molecular mechanisms governing the tuning of Arp2/3 complex nucleated actin filaments, which includes investigations using microfluidics and single-molecule imaging to reveal the mechanosensitivity, dissociation and regeneration of actin branches. We also discuss the high-resolution cryo-EM structure of cortactin bound to actin branches, as well as the differences and similarities between the stability of Arp2/3 complex nucleated branches and linear filaments. These new studies provide a clearer picture of the stabilisation of Arp2/3 nucleated filaments at the molecular level. We also identified gaps in our understanding of how different factors collectively contribute to the stabilisation of Arp2/3 complex-generated actin networks.PMID:38288872 | DOI:10.1042/BST20230638

Source: Biochemical Society Transactions - January 30, 2024 Category: Biochemistry Authors: LuYan Cao Michael Way Source Type: research