Aldehyde Reductase Activity of Carboxylic Acid Reductases

Chembiochem. 2024 Feb 13:e202400121. doi: 10.1002/cbic.202400121. Online ahead of print.ABSTRACTCarboxylic acid reductase enzymes (CARs) are well known for the reduction of a wide range of carboxylic acids to the respective aldehydes. One of the essential CAR domains - the reductase domain (R-domain) - was recently shown to catalyze the standalone reduction of carbonyls, including aldehydes, which are typically considered to be the final product of carboxylic acid reduction by CAR. We discovered that the respective full-length CARs were equally able to reduce aldehydes. Herein we aimed to shed light on the impact of this activity on aldehyde production and acid reduction in general. Our data explains previously inexplicable results and a new CAR from Mycolicibacterium wolinskyi is presented.PMID:38349346 | DOI:10.1002/cbic.202400121

Source: Chembiochem - February 13, 2024 Category: Biochemistry Authors: Margit Winkler Hannah Breuer Lukas Schober Source Type: research