Genes, Vol. 15, Pages 244: miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure

Genes, Vol. 15, Pages 244: miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure Genes doi: 10.3390/genes15020244 Authors: Sung Hwan Cho Young Myeong Kim Hui Jeong An Ji Hyang Kim Nam Keun Kim The primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects experiencing recurrent implantation failure (RIF, n = 5). We conducted transcriptome sequencing and small RNA sequencing on endometrial tissue samples from these cohorts. Subsequently, we validated a selection of intriguing findings using real-time PCR with samples from the same cohort. In total, our analysis revealed that 929 mRNAs exhibited differential expression patterns between the control and RIF patient groups. Notably, our investigation confirmed the significant involvement of dysregulated genes in the context of RIF. Furthermore, we uncovered promising correlation patterns within these mRNA/miRNA pairs. Functional categorization of these miRNA/mRNA pairs highlighted that the differentially expressed genes were predominantly associated with processes such as angiogenesis and cell adhesion. We identified new target genes that are regulated by miR-665, including Blood Vessel Epicardial Substance (BVES) and Adenosylhomocysteinase like 2 (AHCYL...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
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