Molecular characterization of a novel narnavirus infecting the phytopathogenic fungus Botryosphaeria dothidea

AbstractHere, a novel mycovirus, Botryosphaeria dothidea narnavirus 5 (BdNV5), was discovered in the plant-pathogenic fungusBotryosphaeria dothidea strain ZM210167-1. The BdNV5 genome sequence is 2,397 nucleotides (nt) in length and contains a putative open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) with a molecular mass of 72.77 kDa. A BLASTp search using the RdRp amino acid (aa) sequence showed that it was most similar to the RdRp of Botryosphaeria dothidea narnavirus 4 (42.35%). In a phylogenetic tree based on RdRp aa sequences, BdNV5 clustered with members of the familyNarnaviridae. BdNV5 is thus a novel member of the familyNarnaviridae infecting the phytopathogenic fungusB. dothidea.

Source: Archives of Virology - February 1, 2024 Category: Virology Source Type: research