Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

AbstractThe complete genome sequence of a putative novel potyvirus, tentatively named “polygonatum kingianum mottle virus” (PKgMV; GenBank accession no. ON428226), infectingPolygonatum kingianum in China, was obtained by next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE). PKgMV exhibits the typical genome organization and characteristics of members of the genusPotyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (nt 108 to 9,746) encoding a polyprotein of 3,212 amino acids (aa) (363.68 kDa). Pairwise comparisons revealed that the PKgMV polyprotein shares 50.5 –68.6% nt and 43.1–72.2% aa sequence identity with reported members of the genusPotyvirus. Moreover, phylogenetic analysis indicated that PKgMV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKgMV is a novel member of the genusPotyvirus of the familyPotyviridae.

Source: Archives of Virology - February 1, 2024 Category: Virology Source Type: research