Mapping protein–RNA binding in plants with individual-nucleotide-resolution UV cross-linking and immunoprecipitation (plant iCLIP2)

Nature Protocols, Published online: 26 January 2024; doi:10.1038/s41596-023-00935-3RNA-binding proteins orchestrate many aspects of plant development and environmental responses. This protocol describes an optimized plant individual-nucleotide-resolution cross-linking and immunoprecipitation method for genome-wide identification of RNA-binding protein binding sites on their cognate RNAs at single-nucleotide resolution.

Source: Nature Protocols - January 26, 2024 Category: Biomedical Science Authors: Martin Lewinski Mirko Br üggemann Tino K öster Marlene Reichel Thorsten Bergelt Katja Meyer Julian K önig Kathi Zarnack Dorothee Staiger Source Type: research