Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

Nature Protocols, Published online: 24 January 2024; doi:10.1038/s41596-023-00949-xThis protocol describes a method for sampling the microbiome of food-processing facilities and analyzing it by using whole-metagenome sequencing. The protocol includes sampling and DNA-extraction and DNA-purification steps optimized for low-biomass samples.

Source: Nature Protocols - January 24, 2024 Category: Biomedical Science Authors: Coral Barcenilla Jos é F. Cobo-Díaz Francesca De Filippis Vincenzo Valentino Raul Cabrera Rubio Dominic O ’Neil Lisa Mahler de Sanchez Federica Armanini Niccol ò Carlino Aitor Blanco-M íguez Federica Pinto In és Calvete-Torre Carlos Sabater Susana Source Type: research