Structural flexibility of Toscana virus nucleoprotein in the presence of a single-chain camelid antibody

Phenuiviridae nucleoprotein is the main structural and functional component of the viral cycle, protecting the viral RNA and mediating the essential replication/transcription processes. The nucleoprotein (N) binds the RNA using its globular core and polymerizes through the N-terminus, which is presented as a highly flexible arm, as demonstrated in this article. The nucleoprotein exists in an `open' or a `closed' conformation. In the case of the closed conformation the flexible N-terminal arm folds over the RNA-binding cleft, preventing RNA adsorption. In the open conformation the arm is extended in such a way that both RNA adsorption and N polymerization are possible. In this article, single-crystal X-ray diffraction and small-angle X-ray scattering were used to study the N protein of Toscana virus complexed with a single-chain camelid antibody (VHH) and it is shown that in the presence of the antibody the nucleoprotein is unable to achieve a functional assembly to form a ribonucleoprotein complex.

Source: Acta Crystallographica Section D - January 24, 2024 Category: Biochemistry Authors: Papageorgiou, N. Baklouti, A. Lichi è re, J. Desmyter, A. Canard, B. Coutard, B. Ferron, F. Tags: Bunyavirales Toscana virus nucleoprotein flexibility research papers Source Type: research