Molecular characterization of a novel mycovirus from binucleate Rhizoctonia AG-A strain A46

AbstractThe complete genome sequence of a positive-sense single-stranded RNA (+  ssRNA) virus, Rhizoctonia beny-like virus 1 (RBLV1), isolated from binucleateRhizoctonia AG-A strain A46, was determined. The RBLV1 genome is 10,280 nt in length and contains a short stretch of adenines at the 3 ′ terminus. It contains a single open reading frame (ORF) encoding a 376.30-kDa protein with viral helicase and RNA-dependent RNA polymerase (RdRp) motifs. The encoded protein exhibited the highest sequence similarity to Rhizoctonia cerealis beny-like virus 0928-1 (RcBeLV 0928-1, 45.25%), with a s equence coverage of 63%. Phylogenetic analysis based on ORF protein sequences revealed that RBLV1 is a novel unclassified mycovirus.

Source: Archives of Virology - January 18, 2024 Category: Virology Source Type: research