Detection of methandienone and its metabolites in equine urine, plasma and hair following a multidose oral administration

Metabolism and detection of methandienone in urine are well established in horses, but there is limited knowledge about its metabolites in plasma and no information about its detection in hair. Following an oral administration, nine Phase I metabolites were observed in plasma, and the longest detection time was achieved by targeting the parent compound and the mono-hydroxylated metabolite. In hair, the parent compound and hydroxylated metabolites were detected and the mono-hydroxylated offered the longest detection time. AbstractMethandienone is an anabolic-androgenic steroid that is prohibited in equine sports due to its potential performance enhancing properties. Metabolism and detection of methandienone in equine urine have been investigated comprehensively in literature; however, there is a limited knowledge about its metabolites in equine plasma and no information about its detection in equine hair. Following a multi-dose oral administration of methandienone to two Thoroughbred horses, 17-epimethandienone, methyltestosterone, two mono-hydroxylated, two di-hydroxylated and three 17 α-methylandrostanetriol metabolites were detected in plasma. The majority of these were present as free analytes, whilst the mono-hydroxylated metabolites and one isomer of 17α-methylandrostanetriol were partially conjugated. Estimated peak concentrations of methandienone were 6,000 and 11,100 p g/ml; meanwhile, they were 25.4 and 40.5 pg/ml for methyltestosterone. The most abundant analyt...
Source: Drug Testing and Analysis - Category: Drugs & Pharmacology Authors: Tags: RESEARCH ARTICLE Source Type: research