Bioassay-guided isolation and characterisation of α-glucosidase inhibitors from < em > Sanghuangporus baumii < /em >

Nat Prod Res. 2024 Jan 5:1-9. doi: 10.1080/14786419.2023.2300397. Online ahead of print.ABSTRACTBioassay-guided fractionation of the isopropanol extract of the medicinal mushroom Sanghuangporus baumii led to the isolation and characterisation of a new acorane-type sesquiterpenoid bauminene (1) and seven known compounds 2-8. The planar structure of 1 was elucidated on the basis of extensive spectroscopic analysis, including 1D, 2D NMR and HR-ESI-MS. The relative configuration of 1 was determined by a combination of ROESY experiment, density functional theory calculation of 13C NMR, and DP4+ probability analysis, while the absolute configuration of 1 was established by comparative electronic circular dichroism (ECD) spectra analysis. In the in vitro bioassay, compounds 1-8 exhibited potent to moderate α-glucosidase inhibitory activity with IC50 values ranging from 6.8 ± 0.68 to 221.4 ± 6.57 µM. The presences of these bioactive constituents in the sclerotia of S. baumii may be related to the use of the fungus as 'Sanghuang' for the adjuvant treatment of DM.PMID:38179617 | DOI:10.1080/14786419.2023.2300397

Source: Natural Product Research - January 5, 2024 Category: Biochemistry Authors: Xiaoyan Hu Yanling Wang Yuhao Lin Nini Yang Junyi Yao Weiguang Shan Guiwei Rao Youmin Ying Source Type: research