Characteristics of the a sequence of the duck Plague virus genome and specific cleavage of the viral genome based on the a sequence

In this study, we analysed the location, composition, and conservation ofa sequence in the duck plague virus genome. The structure of the DPV genome has ana sequence of (DR4)m-(DR2)n-pac1-S termini (32  bp)-L termini (32 bp)-pac2, and the length is 841  bp. Direct repeat (DR) sequences are conserved in different DPV strains, but the number of DR copies is inconsistent. Additionally, the typical DR1 sequence was not found in the DPVa sequence. ThePac1 andpac2 motifs are relatively conserved between DPV and other herpesviruses. Cleavage of the DPV concatemeric genome was detected, and the results showed that the DPV genome can form a concatemer and is cleaved into a monomer at a specific site. We also established a sensitive method, TaqMan dual qRT ‒PCR, to analyse genome cleavage. The ratio of concatemer to total viral genome was decreased during the replication process. These results will be critical for understanding the process of DPV genome cleavage, and the application of TaqMan dual qRT‒PCR will greatly facilitate more in-depth rese arch.
Source: Veterinary Research - Category: Veterinary Research Source Type: research