Colony and bacterial LAMP: Simple alternative methods for bacterial determination

In this study, we developed colony and bacterial LAMP, which directly use bacterial colony and bacterial culture as the templates without DNA extraction for rapid and simple detection of bacteria. The end-point readouts were determined by naked eye under ultraviolet light, and real-time fluorescence curve was also used to confirm that the sensitivity of this method to Salmonella typhimurium and Bacillus cereus was 102 and 103 CFU/reaction, respectively. Results presented here provide alternative methods for colony and bacterial PCR that can greatly contribute to reliable and cost-effective diagnosis in resource-poor settings.PMID:38161043 | DOI:10.1016/j.ab.2023.115456

Source: Analytical Biochemistry - December 31, 2023 Category: Biochemistry Authors: Lin Zhong Xiaojun Zhang Jiansen Du Junfeng Li Chao Shi Cuiping Ma Yong Li Source Type: research