GSE198470 Mapping primate male germ and somatic cell development using single cell transcriptomics

Contributors : Sara Di Persio ; Sandra Laurentino ; Juan M Paturlanne ; Nina Neuhaus ; Marius W östeSeries Type : Expression profiling by high throughput sequencingOrganism : Callithrix jacchusThe postnatal testicular development is driven by a highly coordinated and fine-tuned series of events. In order to unravel the transcriptional signatures of each developmental stage and identify the potential regulators of this process we performed single cell RNA sequencing. We analyzed testicular cell suspensions obtained from 8 marmoset monkeys (Callithrix jacchus; Common marmoset). Specifically testicular tissues from 3 neonatal (1-2 days old), 3 prepubertal (6 months old) and 2 adult (4-10 years old) were analyzed. 26,341 cells passed quality control filters. In the neonatal we were able to identify, germ cells as well as multiple somatic cell types including Sertoli, Leydig, peritubular myoid, endothelial and immune cells. The same cell types were identified in the prepubertal samples. In the adult, we could largely identify germ cells, with an enrichment of spermatocytes and spermatids.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Callithrix jacchus Source Type: research