High-throughput screening of human mercury exposure based on a low-cost naked eye-recognized biosensing platform

Biosens Bioelectron. 2023 Dec 21;248:115961. doi: 10.1016/j.bios.2023.115961. Online ahead of print.ABSTRACTWhole-cell biosensors could be helpful for in situ disease diagnosis. However, their use in analyzing biological samples has been hindered by unstable responses, low signal enhancement, and growth inhibition in complex media. Here, we offered a solution by building a visual whole-cell biosensor for urinary mercury determination. With deoxyviolacein as the preferred signal for the mercury biosensor for the first time, it enabled the quantitative detection of urinary mercury with a favorable linear range from 1.57 to 100 nM. The biosensor can accurately diagnose urine mercury levels exceeding the biological exposure index with 95.8% accuracy. Thus, our study provided a biosensing platform with great potential to serve as a stable, user-friendly, and high-throughput alternative for the daily monitoring or estimating of urinary mercury.PMID:38150800 | DOI:10.1016/j.bios.2023.115961

Source: Biosensors and Bioelectronics - December 27, 2023 Category: Biotechnology Authors: Bing-Chan Ma Yan Guo Yi-Ran Lin Juan Zhang Xiao-Qiang Wang Wen-Qi Zhang Jin-Gan Luo Yu-Ting Chen Nai-Xing Zhang Qing Lu Chang-Ye Hui Source Type: research