A voltammetric peptide biosensor for Cu < sup > 2+ < /sup > metal ion quantification in coffee seeds

J Inorg Biochem. 2023 Nov 29;251:112441. doi: 10.1016/j.jinorgbio.2023.112441. Online ahead of print.ABSTRACTA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was H-CVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 × 10-8 M detection limit and a 1.0 × 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.PMID:38103419 | DOI:10.1016/j.jinorgbio.2023.112441

Source: Journal of Inorganic Biochemistry - December 16, 2023 Category: Biochemistry Authors: Gabriella Magarelli Jonatas Gomes da Silva Caroline Luchtenberg Ribeiro Thiago Viana de Freitas Magali Aparecida Rodrigues Eric de Souza Gil Pierre Marraccini Jurandir Rodrigues de Souza Clarissa Silva Pires de Castro Marcelo Porto Bemquerer Source Type: research