High-throughput PRIME-editing screens identify functional DNA variants in the human genome

Ren et  al. present PRIME, a genome-scale method to characterize genome sequences by prime editing at the single-base resolution. PRIME enables the analysis of functional DNA elements at the nucleotide resolution by introducing all possible nucleotide substitutions. Additionally, PRIME can be leveraged to characterize disease-associated genetic variations at scale.

Source: Molecular Cell - December 21, 2023 Category: Cytology Authors: Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan, Yun Li, Elad Ziv, Yin Shen Tags: Technology Source Type: research