Why U matters: detection and functions of pseudouridine modifications in mRNAs

Trends Biochem Sci. 2023 Dec 14:S0968-0004(23)00277-3. doi: 10.1016/j.tibs.2023.10.008. Online ahead of print.ABSTRACTThe uridine modifications pseudouridine (Ψ), dihydrouridine, and 5-methyluridine are present in eukaryotic mRNAs. Many uridine-modifying enzymes are associated with human disease, underscoring the importance of uncovering the functions of uridine modifications in mRNAs. These modified uridines have chemical properties distinct from those of canonical uridines, which impact RNA structure and RNA-protein interactions. Ψ, the most abundant of these uridine modifications, is present across (pre-)mRNAs. Recent work has shown that many Ψs are present at intermediate to high stoichiometries that are likely conducive to function and at locations that are poised to influence pre-/mRNA processing. Technological innovations and mechanistic investigations are unveiling the functions of uridine modifications in pre-mRNA splicing, translation, and mRNA stability, which are discussed in this review.PMID:38097411 | DOI:10.1016/j.tibs.2023.10.008

Source: Trends in Biochemical Sciences - December 14, 2023 Category: Biochemistry Authors: Rebecca Rodell Nicolas Robalin Nicole M Martinez Source Type: research