Hi-C sequencing unravels dynamic three-dimensional chromatin interactions in muntjac lineage: insights from chromosome fusions in Fea's muntjac genome

This study aimed to uncover potential genome rearrangement patterns in the threatened species Fea's muntjac (Muntiacus feae), which have not been previously examined for such characteristics. Deep Hi-C sequencing (31.42 × coverage) was performed to reveal the 3D chromatin architecture of the Fea's muntjac genome. Patterns of repeated chromosome fusions that were potentially mediated by high-abundance transposable elements were identified. Comparative Hi-C maps demonstrated linkage homology between the sex chromosomes in Fea's muntjac and autosomes in M. reevesi, indicating that fusions may have played a crucial role in the evolution of the sex chromosomes of the lineage. The species-level dynamics of topologically associated domains (TADs) suggest that TAD organization could be altered by differential chromosome interactions owing to repeated chromosome fusions. However, research on the effect of TADs on muntjac genome evolution is insufficient. This study generated Hi-C data for the Fea's muntjac, providing a genomic resource for future investigations of the evolutionary patterns of chromatin conformation at the chromosomal level.PMID:38017297 | DOI:10.1007/s10577-023-09744-6

Source: Chromosome Research - November 28, 2023 Category: Genetics & Stem Cells Authors: Maryam Jehangir Syed Farhan Ahmad Worapong Singchat Thitipong Panthum Thanyapat Thong Pakpoom Aramsirirujiwet Artem Lisachov Narongrit Muangmai Kyudong Han Akihiko Koga Prateep Duengkae Kornsorn Srikulnath Source Type: research