Expression profiles of lncRNAs, miRNAs, and mRNAs and interaction analysis indicates their potential involvment during testicular fusion in Spodoptera litura

Genomics. 2023 Dec 6:110758. doi: 10.1016/j.ygeno.2023.110758. Online ahead of print.ABSTRACTTesticular fusion of Spodoptera litura occures during metamorphosis, which benefits sperms development. Previous research identified involvement of ECM-integrin interaction pathways, MMPs in testicular fusion, but the regulatory mechanism remains unclear. RNA-seq was performed to analyze long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in testes, aiming to uncover potential regulatory mechanisms of testicular fusion. 2150 lncRNAs, 2742 targeted mRNAs, and 347 miRNAs were identified in testes at three different developmental stages. Up-regulated DElncRNAs and DEmRNAs, as well as down-regulated DEmiRNAs, were observed during testicular fusion, while the opposite expression pattern was observed after fusion. Enrichment analysis of DEmRNAs revealed that cAMP signal pathway, ECM remodeling enzymes, ECM-integrin interaction pathways, and cell adhesion molecules were potentially associated with testicular fusion. The identified DElncRNA-DEmiRNA-DEmRNA regulatory network related to cAMP signal pathway, ECM remodeling enzymes suggests their roles during testicular fusion. Our research will provide new targets for studying the mechanism of testicular fusion.PMID:38065236 | DOI:10.1016/j.ygeno.2023.110758

Source: Genomics - December 8, 2023 Category: Genetics & Stem Cells Authors: Yaqing Chen Yu Chen Xiaoqiang Yu Qili Feng Xiaoyun Wang Lin Liu Source Type: research