< i > SPSL1 < /i > is essential for spermatophore formation and sperm activation in < i > Spodoptera frugiperda < /i >

In this study, our focus was onSPSL1, a gene that encodes a trypsin-type seminal fluid protein inSpodoptera frugiperda, a pest species with global invasive tendencies. Our findings revealed thatSPSL1 expression was predominantly observed in the male reproductive tracts, and the disruption of this gene resulted in male sterility. Surprisingly, fluorescence analysis indicated that the absence ofSPSL1 did not affect spermatogenesis or sperm migration within the male reproductive system. However, when females mated withSPSL1-mutant males, several defects were observed. These included disruptions in spermatophore formation, sperm activation in the copulatory bursae, and sperm migration into the spermathecae. Additionally, mass spectrometry analysis highlighted reduced levels of energy-related metabolites, suggesting thatSPSL1 plays an essential role in promoting hydrolysis reactions during copulation. Consequently, our study demonstrates thatSPSL1 is crucial for male fertility due to its functions in spermatophore formation and sperm activation. This research provides valuable insights into the genetic factors underlying reproductive processes in lepidopteran insects and sheds light on potential strategies for controlling invasive pest populations.
Source: PLoS Genetics - Category: Genetics & Stem Cells Authors: Source Type: research