Delineation of the healthy rabbit tongue by immunohistochemistry - A technical note

Acta Histochem. 2023 Nov 30;126(1):152127. doi: 10.1016/j.acthis.2023.152127. Online ahead of print.ABSTRACTIn the oral cavity the tongue is an important muscular organ that supports the swallowing of food and liquids. It is responsible for the sense of taste, based on the many different taste buds it contains. Research in the field of tongue diseases demands for suitable preclinical models. The healthy rabbit tongue may therefore serve as baseline and reference for the pathological situation. With this consideration, we covered the fixation and histological stainings as well as the immunohistochemical labelling of the healthy rabbit tongue. In this technical note, initial choice of the fixative is discussed, with a comparison of formalin fixation and subsequent paraffin embedding versus cryopreservation. Moreover, we delineate the effect of an antigen retrieval step for formalin fixation by several examples. Finally, we provide ECM markers collagen I, collagen III, fibronectin, α-SMA and elastin staining as well as ki67 for proliferative status and PAR-2 protein expression as a marker for inflammatory status and nociception in tongue sections, mainly from the tongue body. Technically, we found superiority of paraffin sections for collagen I, collagen III, fibronectin, ki67 and α-SMA labelling, for selected detections systems. As for ECM components, the lamina propria was very rich in collagen and fibronectin, while the muscular body of the tongue showed only collagen and f...

Source: Acta Histochemica - December 1, 2023 Category: Biochemistry Authors: Gabriella Meier B ürgisser Dorothea M Heuberger Julia Rieber Iris Miescher Pietro Giovanoli Maurizio Calcagni Johanna Buschmann Source Type: research