De novo Semi-synthetic Platform for Monitoring Protein degradation in Live Cells

Chembiochem. 2023 Nov 30:e202300731. doi: 10.1002/cbic.202300731. Online ahead of print.ABSTRACTWe designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi-synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel-Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post-translationally modified proteins in live cells.PMID:38031893 | DOI:10.1002/cbic.202300731

Source: Chembiochem - November 30, 2023 Category: Biochemistry Authors: Mahdi Hasan Deepanjan Panda Guy Mann Ashraf Brik Source Type: research