A combined top-down and bottom-up LC-HRMS/MS method for the quantification of human growth hormone in plasma and serum

CONCLUSION: The top-down and bottom-up LC-HRMS/MS method developed here presents a valuable tool for the quantification of GH in plasma/ serum complementary to established LBAs used at present in clinical measurements. Albeit the examination of the GH isoform proportions by the LC-MS method does not yet allow for the assessment of GH abuse, the obtained findings provide an important basis to enable LC-MS-based GH analysis of doping control samples in the future.PMID:37995539 | DOI:10.1016/j.ghir.2023.101560
Source: Growth Hormone and IGF Research - Category: Endocrinology Authors: Source Type: research