Using Bland-Altman Analysis to Identify Appropriate Clonogenic Assay Colony Counting Techniques

CONCLUSION: Counting with a manual pen demonstrated significant bias when compared to microscopic and semiautomated colony counts; 2 methods were deemed to be interchangeable. Thus, training algorithms based on manual counts may introduce this bias as well. Algorithms trained to select colonies based on size (pixels2) and shape (circularity) should be prioritized. Solely relying on Bland-Altman or correlational analyses when determining method interchangeability should be avoided; they rather should be used in conjunction.PMID:37997353 | DOI:10.1177/15330338231214250

Source: Technology in Cancer Research and Treatment - November 24, 2023 Category: Cancer & Oncology Authors: Seth Buryska Sanjana Arji Beverly Wuertz Frank Ondrey Source Type: research