ULK1 seen at the single-molecule level during autophagy initiation

Autophagy. 2023 Nov 22. doi: 10.1080/15548627.2023.2286078. Online ahead of print.ABSTRACTMacroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can disturb the inherent balance of cellular components, potentially affecting the integrity of the autophagy process. With the advent of genome-editing techniques like CRISPR-Cas9, it is now possible to tag endogenous proteins with fluorescent markers, enabling the study of their behaviors under more physiologically relevant conditions. Nevertheless, conventional microscopy methods have limitations in characterizing the behaviors of proteins expressed at endogenous levels. This challenge can be overcome by single-molecule localization microscopy (SMLM) methods, which provide single-molecule sensitivity and super-resolution imaging capabilities. In our recent study, we used SMLM in combination with genome editing to explore the behavior of endogenous ULK1 during autophagy initiation, yielding unprecedented insights into the autophagy initiation process.PMID:37992308 | DOI:10.1080/15548627.2023.2286078

Source: Autophagy - November 22, 2023 Category: Cytology Authors: Chiranjib Banerjee Elias M Puchner Do-Hyung Kim Source Type: research